"Hydrocyclone Mammalian Cells"

Effects of hydrocyclones on the integrity of animal and ...

1994-2-1 · The comparative effects of passing mammalian and nucleated red blood cells through liquid/liquid (low shear) and solid/liquid (high shear) hydrocyclones have been examined. Cell disruption was only found when the rig was operated in conjunction with a solid/liquid hydrocyclone apparently as a result of the higher operating pressures required ...

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Hydrocyclone for mAb production in a perfusion single …

2020-10-20 · For the first time, a hydrocyclone specially designed for mammalian cell separation is reported to enable perfusion processes at cell densities in the range of 20 to 50 million/mL for 20 to 25 days. Also for the first time, a HC is connected to a single-use bioreactor.

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The Use of Hydrocyclones for Mammalian Cell Retention in ...

Abstract. A new type of hydrocyclone was designed and used in continuous mammalian cell cultivation processes. The apparatus gave cell separation efficiencies as high as 99%. Due to the fact that a 5-L-bioreactor was used, a sub-optimal configuration of overflow and underflow diameters was used in order to obtain high cell viabilities combined ...

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Numerical simulation of hydrocyclones for cell separation

Figure 1. Perspective view of a hydrocyclone showing the fluid flow inside the equipment (a) and a schematic view showing its geometrical variables (b) whose definitions can be found in the item III.A. A possible new application of hydrocyclones is …

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(PDF) Separation of CHO cells using hydrocyclones

The overflow stream from the hydrocyclone, which is only partially depleted of cells, provides a continuous source of high viability cells from the N‐1 perfusion bioreactor to the 5 to 20 times ...

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(PDF) Separation of CHO cells using hydrocyclones

The overflow stream from the hydrocyclone, which is only partially depleted of cells, provides a continuous source of high viability cells from the N‐1 perfusion …

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Open Source Hydrocyclone Literature Review

2021-3-3 · Abstract Numerical simulation of hydrocyclone aiming at investigating the separation of microorganisms and mammalian cells was performed using Computational Fluid Dynamics (CFD). The turbulence model used in the 2d-axisymmetric calculations was the Reynolds Stress Model (RSM), in order to take into account the high swirl effects that occur in ...

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adjustments to hydrocyclone

Hydrocyclone - Sartorius AG . Hydrocyclone has been developed, designed for the retention of viable mammalian cells. ... For feed flow adjustment an autoclavable pressure gauge is required. KREBS gMAX Cyclones, millMAX Pumps & Technequip Valves . adjustment, which minimizes ... FL Krebs is the leading provider of hydrocyclone technology.

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Complete Upstream Solutions BIOSTAT Bioreactor …

2015-6-25 · – Cell culture – growth of animal, insect and plant cells – Transition from shake or tissue culture fl asks – Small-scale protein expression Applications – Growth studies of microbial, mammalian, insect and plant cells – Culture media optimization – Clone screening – Multivariate process optimization studies (DoE)

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Application of hydrocyclones for continuous cultivation of ...

2011-11-22 · - Hydrocyclone can be successfully used for cell separation in continuous mammalian perfusion bioreactors. - Cells adapt themselves to the shear forces inside the HC without being adversely affected by the pressure. - Higher separation efficiencies up to 96% can be achieved depending on the operating HC pressure.

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Separation of CHO cells using hydrocyclones | SpringerLink

2007-11-14 · The best performance was obtained with a 10-mm Dorr-Oliver hydrocyclone, resulting in a separation efficiency of 81%. These experimental values agree with computational fluid dynamics (CFD) predictions that high levels of separation efficiencies for mammalian cells could be achieved with small-diameter hydrocyclones (Medronho et al. 2005).

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Application of hydrocyclones for continuous cultivation of ...

2011-11-22 · Hydrocyclone can be successfully used for cell separation in continuous mammalian perfusion bioreactors. Cells adapt themselves to the shear forces inside the HC without being adversely affected by the pressure. Higher separation efficiencies up to 96% can be achieved depending on the operating HC pressure.

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Hydrocyclones as cell retention device for CHO perfusion ...

2020-3-17 · In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary (CHO) cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size in ...

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Use of Hydrocyclones for Mammalian Cell Retention ...

A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow ...

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Effect of Crude Oil Particle Elasticity on the Separation ...

2013-3-1 · investigate the separation of microorganisms and mammalian cells. In their study, the presence of a cylindrical air core caused by a low pressure central portion and occupying the whole length of hydrocyclone was observed. In general, two important stages can be distinguished in a separation process; namely, i) separation of particles from the

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Application of hydrocyclones for continuous cultivation of ...

2006-9-6 · A hydrocyclone with a volume of 2.56 cm 3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops of 0.4 to 1.3 bar) the hydrocyclone was characterized with regard to flow split (underflow‐to‐overflow ratio) and flow ratio (underflow to supply).

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A two-step microengineered system for high-density cell ...

2021-1-1 · The use of hydrocyclones for mammalian cell separation has previously been shown as well,,, ... Finally, to assess the performance of the hydrocyclone at higher cell densities, as required by the biomanufacturing industry, the device was tested at cell densities up to 40 × 10 6 cells/mL (Fig. 3B).

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Application of Hydrocyclone for Cell Separation in ...

2005-9-29 · In the present work, the application of a hydrocyclone for cell separation in continuously perfused cultures of mammalian cells was investigated. The characterization of the hydrocyclone performance showed, that a pressure range of 0.85 to 1.40 bar is most suitable to achieve a reliable and stable separation process. Secondly, the effect of hydrocyclone …

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Applications of Membrane Hydrocyclone Recovery of …

2021-7-16 · Recently, [11] used CFD studies to simulate the separation of microorganisms and mammalian cells with hydrocyclones. Some works on the simulation of hydrocyclones using the incompressible Navier -Stokes equations, supplemented by a suitable turbulence model, have proven to be appropriate for modeling the flow in a hydrocyclone [12-13]. 2.

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Very high density of CHO cells in perfusion by ATF or TFF ...

2013-2-22 · Introduction. Although batch and fed-batch processes are the most widely used production systems for mammalian cells, perfusion processes are gaining interest. 1 Perfusion mode provides a constant environment favorable to the cells by continuous by-product removal and nutrient addition. 2-5 In comparison to batch and fed-batch modes, the perfusion mode …

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Application of hydrocyclone for cell separation in ...

Application of Hydrocyclone for Cell Separation in Mammalian Cell Perfusion Cultures Vom Fachbereich für Biowissenschaften und Psychologie der Technischen Universität Carolo-Wilhelmina zu Braunschweig zur Erlangung des Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigte Dissertation von Elsayed Ahmed Elsayed Ahmed aus Kairo, Ägypten 1.

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Hydrocyclones as cell retention device for CHO perfusion ...

2020-3-17 · In this study, a hydrocyclone (HC) especially designed for mammalian cell separation was applied for the separation of Chinese hamster ovary cells. The effect of key features on the separation efficiency, such as type of pumphead in the peristaltic feed pump, use of an auxiliary pump to control the perfusate flow rate, and tubing size in the ...

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Method for separating viable cells from cell suspensions ...

The cells that are separated from cell suspensions, or whose concentration is increased, by the method according to the invention are, for example, mammalian cells, such as blood cells or hybridoma cells, or insect cells. In the Figures, FIG. 1 shows, in principle, the structure of a hydrocyclone used in the method according to the invention;

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Animal Cell Technology Meets Genomics

CGMP Manufacturing of a Fusion Protein in Mammalian Cells Using a Large-Scale Acoustic Perfusion System. Pages 671-674. Crowley, John (et al.) Preview. Influence of Culture Parameters on Viability in Perfusion Process. Pages 675-677. Dalm, Mcf (et al.) Preview. Use of Hydrocyclone as an Efficient Tool for Cell Retention in Perfusion Cultures ...

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Use of Hydrocyclones for Mammalian Cell Retention ...

2006-8-1 · A hydrocyclone with a volume of 2.56 cm3 was studied as a potential cell retention device for mammalian cell cultures (6 L volume). For the feasible operation range (0.9 to 1.6 L/min flow corresponding to pressure drops of 0.4 to 1.3 bar) the hydrocyclone was characterized with regard to flow split (underflow‐to‐overflow ratio) and flow ratio (underflow to supply). Cultures …

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Separation of CHO cells using hydrocyclones ...

2007-11-14 · Hydrocyclones are simple and robust separation devices with no moving parts. In the past few years, their use in animal cell separation has been proposed. In this work, the use of different hydrocyclone configurations for Chinese hamster ovary (CHO) cell separation was investigated following an experimental design. It was shown that cell separation efficiencies for …

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"Hydrocyclones for single-use perfusion application" by ...

Hydrocyclones (HC) are very compact devices that promote solid-liquid separation under the action of a centrifugal field. Despite the small size, HCs have a large processing capacity and do not suffer from clogging. Therefore, several publications explored HCs as a potential cell retention device in perfusion applications in the last 20 years, but limited to non-disposable lab-scale ...

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1. Curriculum Vitae Dr. Elsayed Ahmed Elsayed

2017-12-2 · Advantages of hydrodynamic cell separation in industrial cell culture processes. In: Noll T (Ed.), Cells and Culture. Dordrecht, Springer, ISBN 978-90-481-3419-9, Vol. 4(6): 657-664. Elsayed EA, Piehl GW, Nothnagel J, Medronho RA, Deckwer WD and Wagner R. 2005. Use of hydrocyclone as an efficient tool for cell retention in perfusion cultures.

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(PDF) Separation of CHO cells using hydrocyclones ...

Separation of CHO cells using hydrocyclones. Rodrigo Pinto. Related Papers. Use of Hydrocyclones for Mammalian Cell Retention: Separation Efficiency and Cell Viability (Part 1) ... By R. Medronho. Characterizing hydrocyclone performance for grit removal from wastewater treatment activated sludge plants. By Alain Gadbois.

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Application of Hydrocyclone for Cell Separation in ...

The present work was carried out to investigate the possibility of hydrocyclone application for cell separation in mammalian cell perfusion cultures. Firstly,...

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Size Separation of Rubber Particles from Natural Rubber ...

2010-7-14 · of microorganisms and mammalian cells. In that study, the presence of a cylindrical air core caused by the low pressure central portion occupying the whole length of hydrocyclone was observed. In separation process, two important stages can be distinguished, i.e.,

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